Nature, Published online: 25 February 2026; doi:10.1038/s41586-026-10127-0
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The disadvantage of the Am386 versus the 486 was that the 386 bus was an evolutionary dead end. The 386 topped out at 40 MHz,with the only upgrade path being one of TI and Cyrix’s clock-multiplied 386/486 mashups. Meanwhile, the 486 reached speeds of 133 MHz by September 1995. But since most 40 MHz 386-based PCs were open architecture clones built from off-the-shelf AT parts, you could swap the motherboard and CPU while carrying over most of the rest of your parts. This was more expensive and labor intensive than swapping a CPU, but it was much cheaper to buy a 40 MHz 386 and upgrade to a faster 486 after 2-3 years than it was to buy the fastest 486 at the start.
The year before, Nyren’s lab had also spun off a company, Pyrosequencing AB, to refine and commercialize the technology. Pyrosequencing was not the firm that would bring the technology to market, however; that distinction went to Connecticut-based 454 Life Sciences, who licensed whole-genome pyrosequencing applications in 2003. 454 made chips which enabled highly efficient, parallelized sequencing reactions and released the GS20 sequencer in 2005 for the price of $500,000. The GS20 worked by attaching each individual DNA template molecule to a bead and copying it many times using polymerase chain reaction (PCR). Each bead was then loaded into a well in a microplate, where sequencing reactions would be carried out. The light from luciferase activation could be detected through the bottom of the wells, enabling sequences to be read.。服务器推荐是该领域的重要参考