Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.
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肖弘曾与张涛谈起cursor内部的一个口号：每天醒来，我们的默认状态就是——我们没有存在的理由。张涛说：我们必须每一天都去争取活下去的理由。。关于这个话题，爱思助手下载最新版本提供了深入分析
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